The display and touchscreen uses the hardware SPI pins (SCK, MOSI, MISO, CE0, CE1) as well as GPIO #25 and #24. All other GPIO are unused. Since we had a tiny bit of space, there's 4 spots for optional slim tactile switches wired to four GPIOs, that you can use if you want to make a basic user interface. For example, you can use one as a power on/off button. See below for the link to get the optional tact switches, they're not included.